Correlation Between eAG Values Calculated from Hba1c Values Obtained from Three Different Techniques and Fasting Plasma Glucose Levels for Diagnosis and Prognosis of Diabetes

Abstract

The objective of the present study was to correlate estimated average glucose (eAG) values calculated from HbA1c values obtained from Tosoh TLC-723 G8 based on cation exchange based HPLC, TBA 12 FR enzymatic fructosyl peptide oxidase (FPOX) method and D10 HbA1c analyser Bio Rad Laboratories (USA) with fasting plasma glucose (FPG) levels by hexakinase method for diagnosis and prognosis of diabetes.

A total of 300 randomly selected patient samples were analysed for FPG by hexakinase method and HbA1c values were estimated by using Tosoh TLC-723 G8 analyser based on cation exchange HPLC, enzymatic FPOX (Manufacture by Seikisui Medical Co. Ltd., Japan)  programmed on TBA 12 FR biochemistry analyser and D10 HbA1c analyser Bio Rad Laboratories (USA). The eAG values were calculated using Nanthan’s regression equation for both HbA1c values. The correlation between FPG, HbA1c and eAG values has been done by Pearson correlation (r) and regression analysis was done by SPSS software.

The correlation was good in entire data and poor in differentiated groups. Male data has shown good correlation between FPG and eAG as compared to female data with higher mean levels of FPG, eAG and HbA1c values. Both HPLC methods have shown closer correlation between eAG values calculated from it and FPG values from which Tosoh HLC-723 G8 has shown closer correlation.

The overall correlation between eAG and FPG was good in entire data but mean value difference was found to be slightly high. So for diabetes diagnosis, HbA1c values along with calculated eAG value along with FPG values are recommended as per the American Diabetes Association (ADA) guidelines. As both the HPLC methods have shown good correlation between eAG and FPG values, we recommend that HbA1c values and eAG values calculated from it has to be used for diagnosis and prognosis of diabetes along with FPG values and other relevant clinical findings of patients. Tosoh HLC-723 G8 HPLC HbA1c analyser could be preferred technique for HbA1c estimation due to closer correlation with FPG values and true separation of labile and stable fraction of HbA1c. The use of HbA1c values alone for diagnosis and prognosis of diabetes still need multicentre studies with large sample size in different population.

Keywords: estimated average glucose (eAG), HbA1c, fasting plasma glucose (FPG), diabetes

Cite this Article

Kadhane O, Mukundan U, Malvankar S et al. Correlation Between eAG Values Calculated from Hba1c Values Obtained from Three Different Techniques and Fasting Plasma Glucose Levels for Diagnosis and Prognosis of Diabetes. Research & Reviews: A Journal of Bioinformatics. 2016; 3(3): 1–14p.