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Hairy Root Culture for Mass Production of Medicinal Important Compound Neoandrographolide from Andrographis lobelioides Nees

Samydurai P, A Rajendran

Abstract


Abstract

The present study was focused to develop hairy root culture of Andrographis lobelioides for the production of Neoandrographolide using Agrobacterium rhizogenes (8196), strains for leaf and root explants. The results indicated that the leaf and root explants induced hairy roots formation and enhanced secondary metabolites Neoandrographolide and related compounds. The medium was supplemented with half strength liquid MS medium with 2.0 μM/l Indole butyric acid (IBA). The highest percentage of Neoandrographolide (161.89%) was observed by HPLC. The integration of T-DNA proportion of A. rhizogenes in hairy roots was confirmed by polymerase chain reaction analysis with specific primer of rol C gene plasmid and fragment length of the transferred gene was 540 bp and 480 bp were determined from Ri plasmids of Agrobacterium rhizogenes.

 

Keywords: Agrobacterium rhizogenes, hairy roots, elicitor, Neoandrographolide

Cite this Article

Samydurai P, Rajendran A. Hairy root culture for mass production of medicinal important compound Neoandrographolide from Andrographis lobelioides Nees. Research & Reviews: A Journal of Biotechnology. 2018; 8(2): 14–19p


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References


Benhamou N. Elicitor-induced plant defense pathways. Trends Plant Sci. 1996; 1: 233–240.

Shanks JV, Morgan J. Plant ‘hairy root’ culture. Current Opinion in Biotechnology. 1999; 10: 151-155

Zolala J, Farsi M, Gordan HR, Mahmoodnia M. Producing a High Scopolamine Hairy Root Clone in Hyoscyamus muticus through Transformation by Agrobacterium rhizogenes. J. Agric. Sci. Technol. 2007; 9: 327-339.

Samydurai P, Rajendran A. Ethnobotanical knowledge of threatened plant species Andrographis in Nilgiris biosphere reserve, Tamil Nadu, India. International Journal of Herbal Medicine, 2017; 5(6): 103-107.

Zhao HY, Frang WY. Antithrombotic effect of Andrographis paniculata Nees in preventing myocardial infarction. Chi. Med. Journal. 1991; 104: 770–775.

Kumar RA, Sridevi N, Kumar V, Nanduri S, Rajagopal S. Anticancer and immunostimulatory compounds from Andrographis paniculata. Journal of Ethnopharmacology, 2004; 92: 291-295.

Calabrese C, Berman SH, Babish JG, Ma X, Shinto L, et al. A phase I trial of andrographolide in HIV positive patients and normal volunteers. Phytotherapy Research, 2000; 14: 333-338.

Priyanka RR, Rathod VK. Rapid extraction of andrographolide from Andrographis paniculata Nees by three phase partitioning and determination of its antioxidant activity. Biocatalysis and Agricultural Biotechnology, 2015; 4(4): 586-593.

Nagalekshmi R, Aditya M, Dhanya KC, Krishnan Nair CK. Hepatoprotective activity of Andrographis Paniculata and Swertia Chirayita. Food and Chemical Toxicology, 2011; 49: 3367–3373.

Vetriselvan S, Subasini U. Hepatoprotective Activity Of Andrographis paniculata. International Journal of Research in Pharmaceutical and Nano Sciences. 2012; 1(2): 307- 316.

Zhang XF, Tan BK. Anti-diabetic property of ethanolic extract of Andrographis paniculata in streptozotocin-diabetic rats. Acta Pharmacol Sin, 2000; 21(12):1157-64.

Akhtar MT, Bin Mohd Sarib MS, Ismail IS, Abas F, Ismail A, Lajis NH, Shaari K. Anti-diabetic activity and metabolic changes induced by Andrographis paniculata plant extract in obese diabetic rats. Molecules, 2016; 21 (8); doi: 10.3390/molecules21081026.

Mariya John KM. Exploration of in vitro culture of tea (Camellia spp.) for the production of secondary metabolites. Ph.D Thesis, Bharathiar University, Coimbatore, 2007.

Bulgakov VP, Tchernoded GK, Mischenko NP, Khodakovskaya MV, Glazunov VP, Radchenko SV, Zvereva EV, Fedoreyev SA, Zhuravlev YN. Effect of salicylic acid, methyl jasmonate, ethephon and cantharidin on anthraquinone production by Rubia cordifolia callus cultures transformed with the rol B and rol C genes. Journal of Biotechnology, 2002; 97: 213-21.

Rao RS, Ravishankar GA. Plant cell cultures: Chemical factories of secondary metabolites. Biotechnol. Adv. 2002; 20: 101-153.

Arpita Mahobia, Zenu Jha. Root Cultures: In vitro conservative method for metabolite extraction from A. paniculata. Int.J.Curr.Microbiol.App.Sci. 2018; 7(3): 2442-2450.

Vakil MA, Mendhulkar VD. Enhanced synthesis of andrographolide by Aspergillus niger and Penicillium expansum elicitors in cell suspension culture of Andrographis paniculata (Burm. f.) Nees. Botanical Studies, 2013; 54: 49.

Samydurai P, Ramakrishnan R, Thangapandian V. Agrobacterium rhizogenes mediated hairy root culture and genetic transformation of an endangered medicinal plant of Decalepis hamiltonii Wight & Arn. Journal of microbiology, biotechnology and food science, 2013; 3(3): 191-164.

Rahnama H, Hasanloo T, Reza Shams M, Sepehrifar R. Silymarin production by hairy root culture of Silybum marianum (L.) Gaertn. Iranian Journal of Biotechnology, 2008; 6(2): 113-118.

Verpoorte R, Contin A, Memelink J. Biotechnology for the production of plant secondary metabolites. Phytochem Rev. 2002; 1: 13–25.

Gangopadhyay M, Chakraborty D, Battachary S, Battachary S. Regeneration of transformed plants from hairy roots of Plumbago indica. Plant Cell Tissue and Organ Culture, 2010; 102 (1): 109-114.




DOI: https://doi.org/10.37591/(rrjobt).v8i2.162

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