CRISPR/CAS9 Technology and its Application in Food

Authors

  • Sofiya . Student, Department of Biotechnology, Chandigarh University, Mohali, Punjab, India

DOI:

https://doi.org/10.37591/(rrjobi).v8i2.1174

Keywords:

CRISPR, CAS9, palindromic repeats, gene editing, knockout gene, food biotechnology, gene regulation

Abstract

Advances in the editing technology of genome or genes and also availability for the genome sequence for several crops has developed possibility for the researcher to precisely target the desired phenotype. Such therapy is possible due to CRISPR editing technology, which involves various cloning methods. After proving this concept in certain plants or crops which were used for the demonstration, thus targeting and improving the specific working of CRISPR/CAS9 technology. So, the present article describes the availability of source to plant biotechnologist to determine crop improvement using CRSIPR-CAS9 based gene editing technology or tool.

References

Jansen R, et al. Identification of genes that are associated with DNA repeats in prokaryotes. Mol Microbiol. 2002; 43(6): 1565–1575p.

Adli M. The CRISPR tool kit for genome editing and beyond. Nature Communications. 2018; 9: 1911p.

Barrangou R, et al. Bacteriophage exclusion, a new defense system. EMBO J. 2015; 34 (2): 134–135p.

Huang L, et al. Developing superior alleles of yield genes in rice by artificial mutagenesis using the CRISPR/Cas9 system. The Crop Journal. 2018; 6(5): 475–481p.

Lander ES. The Heroes of CRISPR. Cell. 2016: 164 (1–2): 18–28p.

Dronov R, Howard W. Gene editing and CRISPR. Office of the Chief Scientist. 2017; 14: 5p. Available at: https://www.chiefscientist.gov.au/sites/default/files/

OCS-Gene-Editing-and-CRISPR.pdf

Anshu A, et al. The Rise of the CRISPR/Cpf1 System for Efficient Genome Editing in Plants. Plant Sci. 2020; 11: 264p.

Bortesi L, et al. The CRISPR/Cas9 system for plant genome editing and beyond. Biotechnol Adv. 2015; 33(1): 41–52p.

Jun R, et al. Development and Application of CRISPR/Cas System in Rice. Rice Science. 2019; 26(2): 69–76p.

Endo M, et al. Multigene knockout utilizing off-target mutations of the CRISPR/Cas9 system in rice. Plant Cell Physiol. 2015; 56(1): 41–47p

Liao HK, et al. In vivo target gene activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation. Cell. 2017; 171(7): 1495–1507p. e15.

Belhaj K, et al. Editing plant genomes with CRISPR/Cas9. Curr Opin Biotechnol. 2015; 32: 76–84p.

Hsu PD, et al. Development and applications of CRISPR-Cas9 for genome engineering. Cell. 2014; 157(6): 1262–1278p.

Shew AM, et al. CRISPR versus GMOs: Public acceptance and valuation. Global Food Security. 2018, 9: 71–80p.

Published

2021-10-12

Issue

Section

Review Article