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Green Synthesis and Characterization of Silver Nano-conjugates Using Heliotropium indicum and Glycosmis pentaphylla Leaf Aqueous Extracts

Pranabesh Ghosh, Sohini Kulavi, Subhadra Nandi, Titav Sengupta, Maitrayee Biswas, Prakriti Das, Chandrima Das, Sirshendu Chatterjee

Abstract


Heliotropium indicum Linn. (Family Boraginaceae), an annual herbaceous medicinal plant, is usually known as Indian heliotrope. Glycosmis pentaphylla (Retz.) DC (Family Rutaceae) is a perennial shrub, commonly known as orange-berry. These traditionally used medicinal plants originate from tropical and temperate parts of the world along with India, Southeast Asia, Northern Australia, and some other African countries. Extensive literature studies suggested that the different parts of the plant are reported to have vast medicinal properties. The present research study deals with phytochemical screening and evaluation of antioxidant activity of Heliotropium indicum (HI) and Glycosmis pentaphylla (GP) leaf aqueous extracts, and synthesis of silver nanoconjugates in green route using those extracts. The synthesized nanoconjugates were characterized using UV-Vis, DLS, and FT-IR techniques. Finally, antimicrobial activity of the nanoconjugates was evaluated. Results highlighted that Heliotropium indicum has more phenolics (131.63±4.05 mg GAE/g of dry plant material) and flavonoids (50.79±0.98 mg QE/g of dry plant material) content as well as it was showed higher free radical scavenging activity (77.81±0.52%) than Glycosmis pentaphylla. It was observed that GP-conjugated silver nanoparticles showed antibacterial properties against gram-negative bacteria Escherichia coli (zone of Inhibition=11 mm) and gram-positive bacteria Staphylococcus aureus (zone of Inhibition=2.5 mm), as evident from Kirby-Bauer Disc Diffusion Assay. Though phytochemical concentration was higher in HI with respect to GP, but GPAgNPs showed only antimicrobial activity.

Keywords: Antimicrobial, antioxidants, flavonoids, Glycosmis pentaphylla, Heliotropium indicum, nanoconjugates, phenolics


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References


Nadaroglu H, Azize AG, Selvi I, et al. Green Synthesis and Characterization of Platinum Nanoparticles Using Quail Egg Yolk. Spectrochim Acta A Mol Biomol Spectrosc. 2016; 172: 43 47p.

Ghosh P, Das P, Das C, et al. Morphological Characteristics and Phyto-Pharmacological Detailing of Hatishur (Heliotropium indicum Linn.): A Concise Review. J Pharmacogn Phytochem. 2018; 7(5): 1900 1907p.

Sreejith PS, Praseeja RJ, Asha VV. A Review on the Pharmacology and Phytochemistry of Traditional Medicinal Plant, Glycosmis pentaphylla (Retz.) Correa. JPR. 2012; 5(5): 2723 2728p.

Sahayaraj K, Rajesh S. Bionanoparticles: Synthesis and Antimicrobial Applications, In: Mendez-Vilas A. editor. Science against Microbial Pathogens: Communicating Current Research and Technological Advances. Madrid: Formatex Research Center; 2011.

https://www.nature.com/subjects/nanomedicine

Lee SH, Jun BH. Silver Nanoparticles: Synthesis and Application for Nanomedicine. Int J Mol Sci. 2019; 20(4): pii: E865.

Zhang XF, Liu ZG, Shen W, et al. Silver Nanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeutic Approaches. Int J Mol Sci. 2016; 17(9): pii: E1534.

Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteau Reagent. Methods Enzymol. 1999; 299: 152 78p.

Zhishen J, Mengcheng T, Jianming W. The Determination of Flavonoid Contents in Mulberry and their Scavenging Effects on Superoxide Radicals. Food Chem. 1999; 64(4): 555 559p.

Shen Q, Zhang B, Xu R, et al. Antioxidant Activity in vitro of Selenium-Contained Protein from the Se-Enriched Bifodobacterium Animalis 01. Anaerobe. 2010; 16(4): 380 386p.

Ammal RM, Vijistella GB. Green Synthesis of Silver Nanostructures against Human Cancer Cell Lines and Certain Pathogens. IJPCBS. 2014; 4(1): 101 111p.

Ahmed S, Saifullah, Ahmad M, Swami BL, et al. Green Synthesis of Silver Nanoparticles Using Azadirachta indica Aqueous Leaf Extract. Journal of Radiation Research and Applied Sciences. 2015; 9(1): 1 7p.

Dutta A, Biswas S, Biswas M, et al. Phytochemical Screening, Anti-oxidant and Anti-microbial Activity of Leaf, Stem and Flower of Rangoon Creeper: A Comparative Study. J Med Plants Stud. 2019; 7(2): 123 130p.

Xiugong G, Yourick JJ, Vanessa DT, et al. Toxicogenomic Study in Rat Thymus of F1 Generation Offspring Following Maternal Exposure to Silver Ion. Toxicol Rep. 2014; 2: 341–350p.

Ibrahim HMM. Green Synthesis and Characterization of Silver Nanoparticles Using Banana Peel Extract and Their Antimicrobial Activity against Representative Microorganisms. Journal of Radiation Research and Applied Sciences (JRRAS). 2015; 8(3): 265 275p.

Ghosh P, Das P, Mukherjee R, et al. Extraction and Quantification of Pigments from Indian Traditional Medicinal Plants: A Comparative Study between Tree, Shrub, and Herb. IJPSR. 2018; 9(7): 3052 3059p.

Mukherjee S, Chowdhury S, Ghosh P, et al. Air Pollution has Deep Impact on Plant Pigments: A Comparative Study on Differentially Polluted Areas of West Bengal. Pollut Res. 2018; 37(3): 690 693p.

Banik S, Mukherjee R, Ghosh P, et al. Estimation of Plant Pigments Concentration from Tulsi (Ocimum sanctum Linn.): A Six Months Study. J Pharmacogn Phytochem. 2018; 7(4): 2681 2684p.


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