

Scope of Single cell Genomics: Bioinformatics Review
Abstract
To study the Expression profile of genes and its regulations in single cell resolutions is one of the major factor to improvise the current therapeutic options. Bulk RNA sequencing has been discussed by many groups before, better understanding over analysis methods with standard best practice guidelines were demonstrated extensively in past 2 decades. The single cell resolution of RNA expression data analysis has many varieties of software in open source end. But very less literature were available with respect to 10x softwares. Here we are demonstrating the utility and usage of single cell genomics solution for different application including gene expression, spatial, ATAC and multiome analysis.
Keywords
References
scRNA-tools. The scRNA-tools database is a catalogue of tools for analysing single-cell RNA sequencing da-ta.Available online at: https://www.scrna-tools.org/
Markus Wolfien, Robert David, and Anne-Marie Galow. Single-Cell RNA Sequencing Procedures and Data Analysis (Bioinformatics). Exon Publications; 2021 Mar 20.
Xiaoqing Yu, et al. Statistical and Bioinformatics Analysis of Data from Bulk and Single-Cell RNA Sequenc-ing Experiments. Methods Mol Biol. 2021;2194:143-175.
Jason P. Smith, et al. Analytical Approaches for ATAC-seq Data Analysis. Curr Protoc Hum Genet. 2020 Jun; 106(1): e101.
SEURAT R. toolkit for single cell genomics.Official release of Seurat 4.0.Available online at: https://satijalab.org/seurat/
10 X Genomics. Reveal the full complexity of cellular diversity, cell by cell. Available online at: https://www.10xgenomics.com/products/single-cell-gene-expression
10 X Genomics.Loupe Browser.From data to biological insights. Available online at: https://www.10xgenomics.com/products/loupe-browser
10 X Genomics.What is Cell Ranger? Available online at: https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger
10 X Genomics.What is Space Ranger? Available online at: https://support.10xgenomics.com/spatial-gene-expression/software/pipelines/latest/what-is-space-ranger
10 X Genomics.V(D)J T Cell and B Cell Analysis with cellranger vdj Available online at: https://support.10xgenomics.com/single-cell-vdj/software/pipelines/latest/using/vdj
DOI: https://doi.org/10.37591/(rrjobi).v9i1.1268
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