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Investigations on Microbial Growth Inhibition by Carbon Based Nanomaterials

Pushpa Singh, Smita Shukla

Abstract


We have studied the antimicrobial activity of carbon-based nanomaterials e.g. graphene oxide with their mechanism of action. We have synthesized the graphene oxide using modified Hummer’s method from high purity graphite precursors. The microstructures and morphology of graphene-based materials have been studied using X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), and transmission electron microscopy (TEM). The specific surface area and porosity analysis of graphene oxide are analyzed using Brunauer, Emmett, and Teller (BET) and Barrett, Joyner, and Halenda (BJH) methods to ensure the large surface area access to medicine. We have investigated its antibacterial effect on pathogenic micro-organism by using standard zone of inhibition. A clear inhibition zone was observed with graphene nanoparticles. The standard antibiotics Erythromycin, Streptomycin, and Tetracycline show a smaller zone of inhibition as compared to the nanoparticle-treated disc. The results indicate better inhibition behavior of antibiotics delivered with the graphene nanoparticles. The effect of different concentration would be presented on the basis of obtained results.


Keywords: Antimicrobial activity, graphene oxide, pathogenic micro-organism, zone of inhibition


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References


Hawkey PM, Warren RE, Livermore DM, McNulty CAM, Enoch DA, Otter JA, Peter A, Wilson R. Treatment of infections caused by multidrug-resistant Gram- negative bacteria: report of the British Society for Antimicrobial Chemotherapy/Healthcare Infection Society/British Infection Association Joint Working Party. J. Antimicrobial Chemotherapy. 2018; 73: iii2–iii78p.

Li X, Robinson SM, Gupta A, Saha K, Jiang Z, Moyano DF, Sahar A, Riley MA, Rotello VM. Functional Gold Nanoparticles as Potent Antimicrobial Agents against Multi-Drug-Resistant Bacteria. ACS Nano. 2014; 8 (10): 10682– 10686p.

Ji H, Sun H, Qu X. Antibacterial applications of graphene-based nanomaterials: Recent achievements and challenges. Adv Drug Deliv Rev. 2016; 105: 176-189p.

Kuo WS, Shao YT, Huang KS, Chou TM, Yang CH. Antimicrobial Amino- Functionalized Nitrogen-Doped Graphene Quantum Dots for Eliminating Multidrug- Resistant Species in Dual-Modality Photodynamic Therapy and Bioimaging under Two-Photon Excitation. ACS Appl. Mater. Interfaces. 2018; 10 (17): 14438– 14446p.

Zhang X, Shen J, Zhuo N, Tian Z, Xu P, Yang Z, Yang W. Interactions between Antibiotics and Graphene-Based Materials in Water: A Comparative Experimental and Theoretical Investigation. ACS Appl. Mater. Interfaces. 2016; 8 (36): 24273– 24280p.

Akhavan O, Ghaderi E. Toxicity of graphene and graphene oxide nanowalls against bacteria. ACS Nano. 2010; 4 (10): 5731–5736p.

Li D, Muller MB, Gilje S, Kaner RB, Wallace GG. Processable Aqueous Dispersions of Graphene Nanosheets. Nat. Nanotechnol. 2008; 3: 101-105p.

Park S, An J, Potts JR, Velamakanni A, Murali S, Ruoff RS. Hydrazine-Reduction of Graphite- and Graphene Oxide. Carbon. 2011; 49: 3019–3023p.

Zhang LL, Zhao X, Stoller MD, Zhu Y, Ji H, Murali S, Wu Y, Perales S, Clevenger B, Ruoff RS. Highly Conductive and Porous Activated Reduced Graphene Oxide Films for High-Power Supercapacitors. Nano Lett. 2012; 12: 1806–1812p.

Stankovich S, Dikin DA, Piner RD, Kohlhaas KA, Kleinhammes A, Jia Y, Wu Y, Nguyen ST, Ruoff RS. Synthesis of Graphene-Based Nanosheets via Chemical Reduction of Exfoliated Graphite Oxide. Carbon. 2007; 45: 1558–1565p.

Zhu Y, Murali S, Stoller MD, Ganesh KJ, Cai W, Ferreira PJ, Pirkle A, Wallace RM, Cychosz KA, Thommes M, Su D, Stach EA, Ruoff RS. Carbon-Based Supercapacitors Produced by Activation of Graphene. Science. 2011; 332: 1537– 1541p.

Hu Z, Srinivasan MP, Ni Y. Preparation of Mesoporous High-Surface-Area Activated Carbon. Adv. Mater. 2000; 12: 62-65p.

Memorang. 12-12 AB Susceptibility Testing [Online]. Memorang. Available from https://www.memorangapp.com/flas hcards/133957/12- 12+AB+Susceptibility+Testing/

Balouiri M, Saad MS, Ibnsouda K. Methods for in vitro evaluating antimicrobial activity: A review. J. Pharmaceutical Analysis. 2016; 6: 71-79p.

Sudhakar S, Jaiswal KK, Ramaswamy AP. The Role of Microwave Irradiation Temperature on Nitrogen Doping in Metal-Free Graphene Catalysts for an Efficient Oxygen Reduction Reaction in an Alkaline Condition. ChemistrySelect. 2018; 3: 8962–8972p.

Park S, Lee KS, Bozoklu G, Cai W, Nguyen SBT, Ruoff RS. Graphene oxide papers modified by divalent ions- enhancing mechanical properties via chemical cross-linking. ACS Nano. 2008; 2: 572–578p.

Buchsteiner A, Lerf A, Pieper J. Water dynamics in graphite oxide investigated with neutron scattering. J. Phys. Chem. B. 2006; 110: 22328–22338p.

Kreger BE, Craven DE, McCabe WR. Gram-negative bacteremia. IV. Re- evaluation of clinical features and treatment in 612 patients. Am. J. Med. 1980; 68: 344-355p.


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