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Stability Study of Medhya Churna Used in Treatment of Cerebral Palsy with Respect to Baseline Microbial Diagnostic Modalities

Miral Ramanikbhai Dobariya, M.S. Cholera, K.S. Patel, V.K. Kori


Background: Cerebral Palsy (CP) is the non-progressive static encephalopathy which caused by brain damage and it is the most common motor disability of childhood. Aim: To study of stability of the Medhya Churna with respect to its stability against the microbial contamination. Materials and Methods: The Medhya Churna was prepared and studied to test microbial contamination at regular time intervals. Results: Sample was given for the microbiological examination from the drug preparation to the last date of microbiological study every time. Medhya Churna showed many capsulated gram negative rods arranged singly at the end of 18 days and 47 days of preparation from bag no. 1 and 2 respectively. Rest of bag i.e. 3 to 8 doesn’t show any microbial contamination. Discussion: Sample was stored in airtight plastic container and studied for a period of 361 days. For Medhya Churna analysis of mycological findings and presence of bacteriological findings Wet mount preparation and Gram stain test used respectively. Conclusion: In the microbiological study of Medhya Churna, bag nos. 1 and 2 of Medhya Churna found the microbiological contamination due to lacuna in follow up of sterilization protocol like packaging of drug, hand hygiene during collection of samples. There was no growth found of microorganisms from date 3rd June 2019 till date 8th January 2020, i.e., 8 month-17 days, even in different climate and temperature. Hence in the present study, with respect to microbiological findings, the stability test of Medhya Churna was positive at room temperature, warm and cold, dry and humid conditions.


Cerebral Palsy, Climate conditions, Microbial profile, Medhya Churna, Stability

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Azanha AB, Faria (July 2005). Use of mathematical models for estimating the shelf-life of cornflakes in flexible packaging. Packaging Technology and Science. 18 (4): 161–222. doi:10.1002/pts.686, Available from, Retrieval Date- 17/06/2020.

Ankit Gupta, Mandeep Jayaswal, Pradip Prajapati, Shelf life of Ayurvedic dosage forms-current view, currunt status, and prospective need, Indian Journal of Traditional Knowledge. 0(4):672–677 October 2011.

Ranade Anagha V. Shende, Neuro-pharmacological review on four Medhya dravya described by charaka. IAMJ, Volume 1; Issue 2; March–April 2013.P.1–4.

Reena Kulkarni, K.J. Girish, Abhimanyu Kumar, Nootropic herbs (Medhya Rasayana) in Ayurveda, Journal of Phramacognosy Review, 2012 Jul-Dec; 6(12): 147–153. doi: 10.4103/0973– 7847.99949.

Alfred E Brown (2001), Benson: Microbiological Application, 8th Edition, the McGraw – Hill Companies, p. 64.

Sushruta, Sushruta Samhita, Sutra Sthana, Adhyaya 37/18, Translated by: Dr. Ambikadatt Shashtri, Chaukhambha Sanskrut Samsthan, Varanasi, 2014, p. 182.

Sharangdhara, Sharangdhara Samhita, Purva Khanda, Paribhasha Adhyaya, 1/48, Translated by: Shree Rasiklal Parikh, Sastu Sahitya Vardhaka Karyalaya, Mumbai, 2012, p. 13.

Anonymous. 2016. The gazette of India. Extraordinary Part (II), Section (3), Subsection (1) no. 561. New Delhi.


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