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Biotechnological Prospective of a Group of Beneficial Extremophiles Isolated from Different Sources

Anupama P. Pathak, Mukundraj G. Rathod, Gautam T. Kamble, Disha M. Basve, Shailesh V. Mahalinge, Hulram G. Ankamwar, Sanjay Chavan, Vyankatesh Mane, Gayatri M. Thakur, Santosh Dhopate, Gangadhar Khule


Extremophiles thrive in terrestrial hot springs, hydrothermal vents, glacial ices, saline lakes, solar saltern and acidic and alkaline niches; moreover a group of the extremophiles may grow in household hazardous waste, solvents containing cleaning and polishing chemicals, heavy metals contaminated sites, or in several other habitats such as aphotic, anoxic, athalassohaline and thalassohaline habitats that were previously considered inhospitable for life. Beside aforementioned extreme niches, extremophiles also dwell in poultry farm waste, salted fish, pickles, and many other food items. Extremophiles exhibit various applications in photographic, food, feed, dairy, detergent, pharmaceutical and leather industries. Bioactive compounds from them such as 2-phenylamine, beta-carotene, lycopene, phytoene, siderophores (iron chelators), organic acids, peptide antibiotics, caspase-1 inhibitors and lot of extremozymes are the topics of investigation amongst the researchers of extremophiles. In this context, we isolated and characterized three extremophiles from poultry farm waste and a salted fish Labeo angra. Based on their phenotypic and microscopic observations and biochemical characters the isolates were identified as Alkalibacillus sp. ASM1, Halobacterium sp. SNA2 and Halobacillus sp. SNA3. These extremophiles can be exploited for alkaline protease, alkaline amylase and pigment production. 


Extremophiles, alkaliphiles, pigment producer, halophiles

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Pabulo Henrique Rampelotto Extremophiles and Extreme Environments Life (Basel). 2013 Sep; 3(3): 482–485. doi: 10.3390/life3030482.

Merino N, Aronson HS, Bojanova DP, Feyhl-Buska J, Wong ML, Zhang S and Giovannelli D (2019) Living at the Extremes: Extremophiles and the Limits of Life in a Planetary Context. Front. Microbiol. 10:780. doi: 10.3389/fmicb.2019.00780. 3. Moschetti G, Aponte M, Blaiotta, G. et al. Characterization of halophilic Archaea isolated from different hypersaline ecosystems. Ann. Microbiol. 56, 119–127 (2006). 4. Pathak AP, Sardar AG, Janaj PC. 2014 Exploring the salted fish for salt stable amylase producing bacteria, Indian J. Mar. Sci. 43(10) 1967–1971. 5. MG Rathod, AP Pathak Lonar soda lake: a natural habitat of the industrially important diverse alkaline protease producers, Research & Reviews: A Journal of Life Sciences. 9 (1), 81–93.

Rathod MG, Pathak AP (2014) Wealth from Waste: Optimized alkaline protease production using agro-industrial residues by Bacillus alcalophilus LW8 and its biotechnological applications. J. Taibah Univ. Sci. 8(4): 307–314, DOI: 10.1016/j.jtusci.2014.04.002)

Rathod MG, Pathak AP (2016) Optimized production, characterization and application of alkaline proteases from taxonomically assessed microbial isolates from Lonar soda lake, India. Biocatal. Agric. Biotechnol. 7(2016) 164–173. DOI: 10.1016/j.bcab.2016.06.002.

Rathod MG, Pathak AP. (2016). Data on optimized production and characterization

of alkaline proteases from newly isolated alkaliphiles from Lonar soda lake, India. Data in Brief. 8(3) 863–866. DOI: 10.1016/j.dib.2016.06.044.

Pathak AP, Rathod MG (2015) A report on thermostable alkaline protease producing bacteria from a terrestrial thermal spring. Indian J. Mar. Sci. 44(7) 1104–1111

Pathak AP, Rathod MG (2013) Production and characterization of alkaline protease by Bacillus pasteurii: a Lonar soda lake isolate. Innov. Res. Chem. 1(1): 22–26.

Rathod MG, Pathak AP (2014) Isolation and identification of alkaline protease producers from selected alkaline habitat. Int. J. Innov. Biol. Res. 3(1): 1–6.

Pathak AP, Rathod MG (2015) Cultivable bacterial diversity of terrestrial thermal spring of Unkeshwar. J. Biochem. Tech. 5(4): 814–818.

Pathak AP, Rathod MG (2014) Exploration of Unkeshwar hot springs in Maharashtra for thermostable amylase producer. Res. Rev. Biosci. 8(7): 269–276.

Pathak AP, Rathod MG A review on alkaline protease producers and their biotechnological perspectives Indian J. Mar. Sci. 47 (6), 1113–1119.

Pathak AP, Rathod MG, Mahabole MP, Khairnar RS. (2020). Enhanced catalytic activity of Bacillus aryabhattai P1 protease by modulation with nanoactivator. Heliyon, 6(6), e04053. /10.1016/j.heliyon.2020.e04053.


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