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 The objective of the present work was to conduct comprehensive biochemical and kinetic analysis of a protease enzyme extracted from two Ayurvedic herbs, namely Shankhpushpi (Convolvulus prostratus) and Mulethi (Glycyrrhiza glabra). The primary focus was to characterize various biochemical aspects of the enzyme, encompassing substrate specificity, optimal pH and temperature conditions, stability, and activation energy. Furthermore, crucial kinetic parameters such as Michaelis-Menten constant (Km) and maximum reaction velocity (Vmax) were determined, aiming to gain insights into the catalytic efficiency and performance of the enzyme. The protease enzyme was successfully isolated and purified from Shankhpushpi and Mulethi herbs, followed by biochemical analysis. The present study provided essential insights into the biochemical nature and kinetics of this protease enzyme derived from Ayurvedic herbs. Understanding these properties is fundamental for potential applications in industries related to protein degradation, enzymatic therapy, and dietary supplements. Further research in this area could explore and harness the full potential of these Ayurvedic herbs and their enzymes for broader biotechnological and biomedical applications. On the commercial basis, 60% of protease enzymes are there in the markets and it is also involved in major industries such as pharmaceutical and medical, food, textile, detergent, and leather. The protease enzymes from plant sources needs to be focus on the biochemical activity and kinetic characteristics such as time course, enzyme concentration, temperature optima, pH optima, temperature stability and pH stability, substrate concentration, activation energy, and temperature coefficient. The present work mainly focused on analyzing the biochemical activity of proteolytic enzymes of two Ayurvedic herbs.


Protease enzyme, Ayurvedic herbs, Biochemical analysis, Kinetic parameters, Biomedical applications.

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