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Antimicrobial Protein Modelling Studies and Biophysical Characterization of Opsin Protein in Leptucapugilator

S. Harshini, S. Haritha, K. Shoba


Members of the Ocypodidae family and subgroup Ucinae, fiddler crabs are common in the world's tropics and subtropics. Leptucapugilator (Fiddler crabs), also known as the Atlantic sand fiddler crab or calling crab, is a species of decapod mollusc that belongs to the Uca family. They are Ocypodidae brachyuran crabs, some of the most recent aquatic organisms to reach the coast. West Africa, the Western Atlantic, the Eastern Pacific, the Indo-Pacific, and the Algarve regions of Portugal are among the places where fiddler crabs can be found. They are readily identifiable by their noticeably asymmetrical claws. These species can be found in mangroves, saline marshes, lagoons and wetlands, brackish intertidal mud flats, sandy or muddy coasts, lagoons, and swamps. Fiddler crabs help with the mineralization and turnover of essential minerals. Numerous species-specific patterns of body colour can be used as intra- and inter-specific connection cues. Understanding the behavioural and ecological significance of this colouring requires knowledge of whether fiddler crabs possess the physiological ability to identify colour cues. The photoreceptor protein opsin, which is present in U. pugilator, serves as a basic component of the molecular machinery responsible for colour perception and possibly trichromatic vision. The biophysical characterization of opsin protein in Leptucapugilator was carried out using a dipole movement server after the antimicrobial protein modelling studies were carried out using AmiGO, T-COFFEE, and the Swiss model.

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Vidal-Gadea AG, Rinehart MD, Belanger JH. Skeletal adaptations for forward and sideways walking in three species of decapod crustaceans. Arthropod Struct Dev. 2008 Mar; 37(2): 179–194. doi:10.1016/j.asd.2007.06.002. PMID 18089130

Adams DC, Collyer A, Kaliontzopoulou A. 2020. Geomorph: software for geometric morphometric analyses. R package version 3.2.1. Accessed 27 January 2020.

Capparelli MV, Abessa DM, McNamara JC. Effects of metal contamination in situ on osmoregulation and oxygen consumption in the mudflat fiddler crab Ucarapax (Ocypodidae, Brachyura). Comp Biochem Physiol C Toxicol Pharmacol. 2016 Jul–Aug; 185–186: 102–111.

Schweitzer Carrie E, Feldmann Rodney M. The oldest Brachyura (Decapoda: Homolodromioidea: Glaessneropsoidea) known to date (Jurassic). J Crust Biol. 2010; 30(2): 251–256.

Tan Leo WH, Ng Peter KL, Fiddler Crab. A Guide to Seashore Life. Singapore: Singapore Science Centre; 1988.

Jocelyn Crane. Fiddler crabs of the world (Ocypodidae: genus Uca). Princeton, New Jersey: Princeton University Press; 2016; 736. Epub 2016 Mar 16.

Guaní-Guerra E, Santos-Mendoza T, Lugo-Reyes SO, Terán LM. Antimicrobial peptides: general overview and clinical implications in human health and disease. Clin Immunol. 2010; 135(1): 1–11.

Guinot Danièle. New hypotheses concerning the earliest brachyurans (Crustacea, Decapoda, Brachyura). Geodiversitas. 2019; 41(1): 747–796. doi:10.5252/geodiversitas2019v41a22

Clark HL, Backwell PRY. Territorial battles between fiddler crab species. R Soc Open Sci. 2017 Jan 18; 4(1): 160621. Affiliations expand, PMID: 28280560

Hale JD, Hancock RE. Alternative mechanisms of action of cationic antimicrobial peptides on bacteria. Expert Rev Anti Infect Ther. 2007; 5(6): 951–959.

Hsi-Te Shih, Joseph Poupin. A New Fiddler Crab of AustrucaBott, 1973, Closely Related to A. perplexa (H. Milne Edwards, 1852) (Crustacea: Brachyura: Ocypodidae), from the South Pacific Islands. Zool Stud. 2020 Jul 6; 59: e26.

Klingenberg CP, Barluenga M, Meyer A. Shape analysis of symmetric structures: quantifying variation among individuals and asymmetry. Evolution. 2002; 56(10): 1909–1920. doi:10.1111/j.0014-3820.2002.tb00117.x. [PubMed]

Lanfear R, Frandsen PB, Wright AM, Senfeld T, Calcott B. Partition Finder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Mol Biol Evol. 2017; 34(3): 772–773. doi:10.1093/molbev/msw260. [PubMed]

Mangale VY, Kulkarni BG. Biodiversity of Fiddler Crabs in Mumbai Region. Trends Life Sci. 2013; 2(4): 19–21.

Zolkhiflee N, Shuhaida S, Yahya K. Carapace width-weight relationship and condition factor of Austrucaannulipes in mangroves of Penang Island. IOP Publishing. 2020; 736(1–3).

Chen P, Lin AY, McKittrick J, Meyers MA. Structure and mechanical properties of crab exoskeletons. Acta Biomater. 2008 May; 4(3): 587–596. doi:10.1016/j.actbio.2007.12.010. PMID 18299257

Rosa Rafael D, Barracco Margherita A. Antimicrobial peptides in crustaceans. Invertebr Surviv J. 2010; 7: 262–284.

Roy AK, Rumpi Ghosh, Upadhayay AD. In silico analysis, structure modeling and phosphorylation site prediction of vitellogenin protein from Gibelioncatla. J Appl Biotechnol Bioeng. 2017; 3(1): 265–270. DOI: 10.15406/jab,2017.03.00055

Shoba K, Manjuladevi M, Mazher Sultana. Biochemical analysis and gene expression profiling on collagenase protein in fiddler crab. World J Pharm Pharm Sci. 2017; 6(3): 747–756. ISSN 2278 – 4357.

Shoba K, Sowmiya S, Mazher Sultana. Biochemical analysis and homology modeling of collagenase protein of scylla serrata crab. World Journal of Pharmaceutical and Life Sciences. 2017; 3(1): 427–436. ISSN 2454–2229

Shoba K, Hebsibah Elsie B, Bavyasri S. In silico Peptide Modeling Studies and Structural Analysis on Ribulose -1, 5 Bisphosphate Carboxylase in GracilariaEdulis. World J Pharm Pharm Sci. 2018; 7(3): 1086–1095. ISSN 2278–4357.

Shoba K, Kalpana K. Protein Modeling and Drug Docking Studies on Potential Protein Target (E. coli–dosP) and Compound Aldehyde (Sumatriptan) using Bioinformatics Tools. Res Rev: J Bioinform. 2018; 5(3): 9–18.

Shoba K, Hebsibah Elsie B, Jayakumari S, Sathya R. Insilico Structural Analysis and Drug Docking Stuidies On Ribulose -1, 5 Bisphosphate Carboxylase In Gracilaria Edulis. Int J Adv Res. 2018; 6(9): 159–165. (ISSN 2320-5407).

Shoba K, Nithy G, Deepa L. Biochemical Analysis and Peptide Modeling of Lysozyme in Indian Fenneropenaeusindicus shrimp species. Int J Adv Res. 2018; 6(9): 382–388. (ISSN 2320–5407).

Shoba K, Mazher Sultana. Three-dimensional structure and motif prediction studies on collagenase protein in fiddler crab. Int J Novel Trends Pharm Sci. 2016; 6(4): 79–83. ISSN: 2277–2782.

Shoba K, Hebsibahelsie B. In silico homology modeling ofribulose-1, 5bisphosphate carboxylase protein in gracilariaedulis. World J Pharm Pharm Sci. 2017; 6(8): 396–406. ISSN 2278–4357.

Shoba K, Lavanya G. Identification of De Novo Peptide and Motif Prediction on Porphyria Protein (Hmbs) Using Insilico Tools. Int J Novel Trends Pharm Sci, 2016; 6(2), 45-47. Issn 2320–303x.

Shoba K, Lavanya G. Tertiary Structural Prediction and Drug Binding Studies on Mutated Gene (Hmbs) in Human Porphyria. Int J Novel Trends Pharm Sci. 2017; 7(6): 236–240. ISSN 2277–2782

Usha K, Shoba K. Functional analysis & peptide structure modeling of sphyastatin protein in scyllaparamamosain. International Journal of Current Science and Technology. 2019 Jan; 7(01(A)): 666–669. ISSN: 2320–8090.

Uthai Kuhapong, Fahmida Wazed Tina, Kiadtisak Limsakuna, Suranan Watthanaphonga, Ekapote Luckbana, Teethat Piyakuna. Temporal variations in the air, soil and fiddler crab (Austrucaperplexa) burrow temperatures in southern Thailand. J Anim Behav Biometerol. 2021; 9(1): 2113.



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