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Bacterial Infected Diseases on Fish Clarias Batrachus Treated by Nanoparticle

Smita Shukla

Abstract


An experiment was designed to investigate the antibacterial activity of two nanoparticles against fish bacterial pathogens Bacillus subtilis, Vibrio harveyi species. Different concentration of nanoparticles was assessed by well diffusion method against an antibacterial activity. The nanoparticles was analysed by MIC and MBC technique. The potential nanoparticle CeO2 which showed maximum antibacterial was also subjected for the time killed assay method. Among the two nanoparticles CeO2 shows maximum activity against Bacillus subtilis (14+0.45 mm diameter) MIC test carried by the liquid dilution method. The result suggested that the CeO2 nanoparticles showed maximum inhibition at the concentration of 19 µg/ml against Bacillus subtilis and 29 µg/ml against vibrio. It is also noted that 9 µg/ml concentration of CeO2 nanoparticles showed the maximum reduction of bacteria growth 2nd hour up to 12th hours. It is concluded from the present study the CeO2 could be used as an affective antibacterial agent for disease free fish management.


Keywords: Nanoparticles, MBC, MIC, time kill assay


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References


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