Open Access Open Access  Restricted Access Subscription or Fee Access

Bacterial Infected Diseases on Fish Clarias Batrachus Treated by Nanoparticle

Smita Shukla


An experiment was designed to investigate the antibacterial activity of two nanoparticles against fish bacterial pathogens Bacillus subtilis, Vibrio harveyi species. Different concentration of nanoparticles was assessed by well diffusion method against an antibacterial activity. The nanoparticles was analysed by MIC and MBC technique. The potential nanoparticle CeO2 which showed maximum antibacterial was also subjected for the time killed assay method. Among the two nanoparticles CeO2 shows maximum activity against Bacillus subtilis (14+0.45 mm diameter) MIC test carried by the liquid dilution method. The result suggested that the CeO2 nanoparticles showed maximum inhibition at the concentration of 19 µg/ml against Bacillus subtilis and 29 µg/ml against vibrio. It is also noted that 9 µg/ml concentration of CeO2 nanoparticles showed the maximum reduction of bacteria growth 2nd hour up to 12th hours. It is concluded from the present study the CeO2 could be used as an affective antibacterial agent for disease free fish management.

Keywords: Nanoparticles, MBC, MIC, time kill assay

Full Text:



Pandey R, Khuller GK. Nanotechnology based drug delivery system(s) for the management of tuberculosis. Ind J Exp Biol. 2006; 44 (5): 357–366p.

Scott NR. Nanotechnology and animal health. Rev Sci Technol. 2005; 24(1): 425- 432p.

Johari R, Kumar G, Kumar D. Singh S. Synthesis and antibacterial activity of m (ii) Schiff base complex. J Ind Coun Chem. 2009; 26 (1): 23–27p.

Gong P, Li H, X, Wang K, Hu J, Tan W, Zhang S, Yang X. Preparation and antibacterial activity of Fe3O4@Ag nanoparticles. Nanotechnol. 2007; 18:604–611p.

Padmavathy N. Vijayaragluvan R. Enhanced bioactivity of ZnO nanoparticles-an antimicrobials study. Sci Technol. Adr. Mat. 2008; 9(3): 035004.

Rajendran R, Balakumar C, Hasabo AMA, Jayakumar S, Vaideki K, Rajesh EM. Use of ZnO nanoparticles for production of antimicrobial textile. Int J. Eng Sci Technol. 2010; 2(1): C202–208p.

Haritha M, Meena V SCC, Srinivasa RB. Synthesis and characterization of Zinc oxide nanoparticles and its antimicrobial activity against Bacillus subtilis and Escherichia coli. Rasavan J Chem. 2011; 4(1):217–222p.

Kuzma J, Verhage P. Nanotechnology in agriculture and food production: anticipated application. Project on emerging nanotechnologies and the consortium on law, values and health and life sciences. Centre for Science, Technology and Public Policy (CSTPP), 2006.

Rajeshkumar S, Venkatesan C, Sarathi M, Sarathbabuy V, Thomas J, Anver Basha K, Sahul Hameed AS. Oral delivery of DNA construct using chitosan nanoparticles to protect the shrimp from white spot syndrome virus (WSSV). Fish & Shellfish Immunology. 2009; 26: 429– 437p.

Mehdi R, Mahra A. In vitro antimicrobial effect of silver nanoparticles on Lactococcus garvieae and Streptococcus iniae. African Journal of Microbiology Research. 2011; 5(25): 4442–4445p.


  • There are currently no refbacks.