Screening, Isolation and Characterization of Cypermethrin Tolerating Bacteria from Sugarcane Field Soil

Abstract

Cypermethrin is a synthetic pyrethroid pesticide group widely used to control pests in cotton, fruits, and vegetable crops. Excessive use of pesticide in agriculture is one of the major causes of environmental pollution. The present study was aimed to screen, optimize and identify Cypermethrin-tolerant bacteria. For this purpose the pesticide exposed soil samples were collected and bacterial strains were isolated on Cypermethrin-containing Nutrient agar plates. On the basis of physical and chemical characterization, ten isolates were obtained named as KCl to KC10. The isolate KC1 was grown in Nutrient Broth Medium (NBM) at various pH, temperature, NaCl concentrations, carbon source and nitrogen source. The optimum growth pH and temperature of KC1 were found to be pH 7 and 37 °C, respectively. About 3% NaCl concentration was found to be optimum for growth. Optimum growth was obtained while using glucose as a carbon source and beef extract as a nitrogen source.

Keywords: Bacillus, Cypermethrin, degradation, KC 1, pyrethroid, soil

Cite this Article Barot J, Chaudhari K. Screening, isolation and characterization of cypermethrin tolerating bacteria from sugarcane field soil. Research & Reviews: A Journal of Biotechnology. 2018; 8(1): 11–15p.