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A Rapid and Efficient Protocol of DNA Extraction from Animal Blood and Plant Parts

Patnaik Amit, Sharma Hanuman Prasad, Rani Priya, Pandey Kumar Narendra, Anita ., Ranjan Alok

Abstract


DNA extraction from plant tissues is much more complex in comparison to mammalian tissues and bacterial cells due to the presence of a rigid cell wall. Methods utilized for extraction of DNA from plant tissues can vary depending upon the materials used. It is very essential to obtain the nuclear material (without degradation) by breaking down the cell wall and membrane mechanically. We have used the single protocol for extraction of DNA from blood as well as from explants of anti-cancer plants and obtained fruitful results.

 

Keywords: DNA, Blood, Electrophoresis, Extraction Buffer, Agarose, Transilluminator


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