A Rapid in vitro Propagation of Bambusa balcooa by Plant Tissue Culture Technique

Khileshwar Chandra Kumar Sahu, D.S.V.G.K. Kaladhar, Belorkar S. A.

Abstract


Bambusa balcooa is a common plant grown in Bihar, Orissa, Jharkhand and Uttarakhand states of India having good cultural importance. The present experimentation on nodal explants of B. balcooa on MS media supplement under particular culture conditions have shown shunted growth in 30 days. Shoot tip explants of B. balcooa seedlings produced multiple shoots on MS medium supplemented with different plant growth regulators (PGRs) individually and in combination. Shoot tip explants of B. balcooa requires 30 days to initiate shoots. Sub-culturing at lower concentration of Kn (0.25 μg/l, 0.50 μg/l 1.0 μg/l and 1.50 μg/l respectively) fortified medium caused stagnant and unhealthy of the shoots, leading to ultimate death of the cultures. Among the three cytokinins tested, BAP was selected as the most suitable hormone to induce shoot multiplication. Highest shoot multiplication is found by incorporation with BAP (1.5 μg/l and 2 μg/l). The shoot multiplication rates are good with shoot length 2.5±0.3 cm and 3.9±0.4 cm with highest and maximum shoot generation. Shoot regeneration was also achieved in the MS medium containing Kn (1.0, 1.5 and 2.0 μg/l). Highest multiplication is observed in the concentration of Kn (2.0 μg/l). The shoot multiplication rate is moderate with shoot length of 2.00±0.5 cm. Multiplication potentiality was observed in the cluster having more than 2–3 shoots. Best period for recycling of multiplying shoots is 2–3 weeks in old culture. Delaying of sub-culturing period resulted in gradual browning of the shoots. The sub-culturing period was recorded as the most crucial factor for obtaining optimal and desired level of regeneration of shoots. The well grown prop gules in the present experimentation were shown successful regeneration. Hence, the experiment concluded with best growth pattern observed in media containing auxins like BAP and cytokinins like Kinetin.
Keywords: Bambusa balcooa. Roxb, BAP, kinetin, in vitro propagation

Cite this Article

Khileshwar Chandra Kumar Sahu, DSVGK Kaladhar, Belorkar SA. A Rapid in Vitro Propagation of Bambusa Balcooa by Plant Tissue Culture Technique. Research & Reviews: A Journal of Biotechnology. 2018; 8(1): 27–31p.


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