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Performance Evaluation of Various Disinfectant for Inactivation of Coliform Bacteria in Potable Water

Trupti Kekare, Dhananjay Bhatkhande, Tanushree Bhattacharjee, Shraddha Khamparia


Water is important for life, but many individuals do not have access to clean and safe potable water and die because of waterborne diseases.In the present study, laboratory experiments were performed for evaluation of efficiency of various disinfectant for inactivation of coliform bacteria present in water. Ground water (Borewell) was used as sample water for experiment. Chlorine (Calcium hypochlorite and Sodium hypochlorite), Neem seed extract and Heat treatment (Boiling)was used as disinfectant. Different concentration of Calcium hypochlorite and Sodium hypochlorite was treated with water sample for contact time of 30 minutes. Different volumes of neem seed extract were used to treat sample water for 30 minutes. Heat treatment method on sample water was carried out for 15 minutes. Plate count method was used to estimate initial and final number of viable cells (CFU/ml) present in sample water. By using initial and final count of bacteria percentage reduction was calculated. Results showed that, calcium hypochlorite and sodium hypochlorite was able to reduce growth of bacteria to 99 percentage. On the other hand, neem seed extract was able to inhibit growth only up to 90 percentage reduction. Heat treatment(boiling) showed effective reduction in growth of bacteria.


Coliform, Calcium hypochlorite, Sodium hypochlorite, Neem seed extract, Heat treatment

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